Table 2 : Effect of temperature on cytoplasmic protien retention in Sannhemp

Sr. No.	Treatment	Time of incubation in hrs.	NPN / ml	PN / ml	% retention
1.	-	0	0.60	3.01	100
2.	30 to 33°C	1/2	1.20	2.93	97.34
3.	40°C	1/2	1.32	2.81	93.35
4.	60°C	1/2	1.77	2.36	78.40
5.	80°C	1/2	2.45	1.53	50.83
6.	30 to 33°C	3	1.60	2.53	84.05
7.	40°C	3	2.22	1.91	63.45
8.	60°C	3	2.41	1.72	57.14
9.	80°C	3	2.60	1.01	3.55

 

Table 3 : Effect of pH on cytoplasmic protein retention.

Sr. No.	Treatment	pH	Initial NPN/  ml in mg		NPN / ml  after 3hrs.		Initial PN/ ml in mg		PN/ml after  3 hrs in mg		% retention of PN
			L	S	L	S	L	S	L	S	L	S
1.	2		0.65	1.75	1.15	2.02	1.47	2.58	0.97	2.31	68.30	89.53
2.	4		1.09	1.79	1.26	2.03	1.03	2.34	0.86	2.30	60.56	89.14
3.	5.7		0.60	0.60	1.25	2.35	1.52	3.73	0.77	1.89	61.26	73.25
4.	8		1.02	1.50	1.20	2.33	1.10	2.83	0.92	2.00	64.78	77.51
5.	10		1.10	1.72	1.18	1.95	1.02	2.61	0.94	2.38	66.19	92.24

L = Lucerne      S = Sannhemp      NPN = Non protein nitrogen      PN = Protein nitrogen.


Table 4 :Effect of temperature on cytoplasmic protein retention.

Sr. No.	Treatment Temp erature *C	Time of incubation  in hrs	NPN / ml in mg		PN / ml in mg		% retention of PN
			Lucern	Sannhemp	Lucerne	Sannhemp	Lucerne	Sannhemp
1.	-	0	0.60	0.60	1.42	3.01	100	100
2.	30 - 33 (RT )	1/2	0.85	1.20	1.27	2.93	89.43	97.34
3.	40	1/2	1.00	1.32	1.20	2.81	84.50	93.35
4.	60	1/2	1.02	1.77	1.10	2.36	74.46	78.40
5.	80	1/2	1.16	2.45	0.96	1.53	67.60	50.83
6.	30 - 33 (RT )	3	1.20	1.60	0.92	2.53	64.78	84.05
7.	40	3	1.25	2.22	0.87	1.91	61.26	63.45
8.	60	3	1.35	2.41	0.77	1.72	54.22	57.14
9.	80	3	1.75	2.60	0.37	1.01	26.05	33.55

ACKNOWLEDGEMENTS

The authors are grateful to Dr. R. N. Joshi for guidance and Prof. K. B. Deshpande for facilities.

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