Dr. Dilip Boralkar

List of Publications of Dr. Dilip Boralkar

Research Papers Published by DBB

Factors affecting the proteolysis of cytoplasmic protein

Marath. Univ. J. of Sc. (Nat. Sci.) XVIII Sc.II, 25-28, 1979.
D. B. Boralkar and Usha R. Nagpal
Department of Botany, Marathwada University, Aurangabad.

ABSTRACT

Effects of cytokinin, gibberellic acid, vanillin, coumarin, 2-4 Dinitrophenol, Ascorbic acid, indole-3-acetic acid, pH and temperature on retention of cytoplasmic protein were investigated. It was observed that cytokinin, gibberellic acid, indole-3-acetic acid could prevent the breakdown of cytoplasmic protein in similar manner as in the leaf saps. Higher temperatures and acidic pH caused protein degradation, however, retention of cytoplasmic protein was noted at alkaline pH.

INTRODUCTION

Intensive work on agronomy of leaf protein has been done by earlier workers ...

Table 1: Effect of chemicals on cytoplasmic protein retention in Sannhemp

S.N.	Treatment	Initial NPN/ml	NPN/ml after 3 hrs	Initial PN/ml	PN/ml after 3 hrs	% Retention
1	Control	0.60	2.35	3.53	1.78	50.42
2	IAA	-	2.07	-	2.06	58.35
3	AA	-	2.04	-	2.09	59.20
4	Coumarin	-	1.91	-	2.22	62.88
5	2-4 DNP	-	2.08	-	2.05	58.07
6	GA	-	2.06	-	2.07	58.64
7	Kinetin	-	2.16	-	1.97	55.80

Table 2: Effect of temperature on cytoplasmic protein retention in Sannhemp

Sr. No.	Treatment	Time (hrs)	NPN/ml	PN/ml	% Retention
1	-	0	0.60	3.01	100
2	30–33°C	1/2	1.20	2.93	97.34
3	40°C	1/2	1.32	2.81	93.35
4	60°C	1/2	1.77	2.36	78.40
5	80°C	1/2	2.45	1.53	50.83
6	30–33°C	3	1.60	2.53	84.05
7	40°C	3	2.22	1.91	63.45
8	60°C	3	2.41	1.72	57.14
9	80°C	3	2.60	1.01	33.55

ACKNOWLEDGEMENTS

The authors are grateful to Dr. R. N. Joshi for guidance and Prof. K. B. Deshpande for facilities.

REFERENCES

Osborne, D. J. (1962) : Plant Physiol. 37 : 595.
Suiguira et al. (1965) : Growth and development of plants.
Singh, N. (1962) : J. Sci. Fd. Agric. 13 : 315.
Thimann et al. (1970 ) : Plant growth substances.
Thimann et al. (1975) : Plant Physiol. 55 : 828.
Usha Rani Batra (1975) : Ph.D. Thesis.
Usha Rani Batra (1976) : In Jr. Plant Physiol. 19 : 2, 211.

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